Ultrastructural Characterisation of isolate 127 of egg drop syndrome 1976 virus as an adenovirus

Application of polymerase chain reaction in detection of egg drop syndrome (EDS) virus

  Egg drop syndrome (EDS) is caused by a hemagglutinating adenovirus which has become a major cause of lost egg production and sever economic losses in breeder and layer chicken flocks throughout the world. A PCR assay was optimized for detection of EDS virus in inoculated allantoic fluids (AFs) of duck and chicken emberyonated eggs. Two strains of EDS viruses were propagated in allantoic cavit...

Studies on Egg Drop Syndrome in Iran

Incidence of egg drop syndrome

Aim: To know the magnitude of influence by Egg Drop Syndrome – 1976 (EDS –'76) virus infection in causing drop in egg production in and around Namakkal. Materials and Methods: A total of 150 cloacal swabs and 15 pouch shell glands (uteri) homogenates from 15 poultry farms in and around Namakkal area were used for virus isolation. Three numbers of 10 –dayold embryonated duck eggs were used for t...

Preparation of an Inactivated Egg Drop Syndrome Antigen for Using in Hemagglutination Inhibition Test.

To prepare hemagglutination inhibition (HI) antigen the egg drop syndrome virus (EDSV) was propagated in 10-day-old embryonated duck eggs. The virus antigen was inactivated with two methods including heating (65°C) and adding 0.5% formaldehyde by considering low destroying effect on hemagglutination (HA) and HI titers. The EDSV HI titers of 310 sera and 100 yolks obtained from 23 chicken farms ...

Isolation and identification of adenovirus 127, the causative agent of egg drop syndrome (EDS), from commercial laying hens in South Africa.

A drop in egg production associated with loss of pigment and soft-shelled eggs was experienced on a farm in the Western Cape. Investigations revealed high levels of antibodies against adenovirus 127, the causative agent of egg drop syndrome (EDS) in chickens which were not vaccinated against EDS. A cytopathic agent was subsequently isolated in chicken embryo liver cell cultures and identified a...